Journal: Journal of advanced research
Article Title: Maternal fish oil supplementation enhances placental nutrient transport and mammary gland secretion via the GPR120 signaling pathway.
doi: 10.1016/j.jare.2024.12.029
Figure Lengend Snippet: Figure 5: Effect of DHA on milk fat and milk protein synthesis in PMECs. A:The oil red O staining of PMECs, n=3. The scale is 100 μm. B-C: Triglyceride concentration in cells (B) and in the medium (C), n=6. D-G: Western blot bands of milk fat synthesis- related protein in PMECs, n=3. H: Fluorescence microscopy images of α-casein (green), β-casein (green), WAP (green) and DAPI (blue) in PMECs, n=3. The scale is 200 μm. The small red box indicates the selected area, and the three smaller images on the right show the target protein, nucleus and merged image within the selected area. I-J: Western blot bnads of milk protein in PMECs, n=3. K-L: Western blot bands of JAK2- STAT5 signaling-related protein in PMECs, n=3. M-N: Western blot bands of mTORC1 signaling-related protein in PMECs, n=3. * indicated statistical significance (P < 0.05), ** indicated highly significant differences (P < 0.01) and ns indicated no significant differences (P > 0.05).
Article Snippet: p-P70 S6K1 CST 9234S 1000 4E-BP1 Abcam ab32024 1000 p-4E-BP1 CST 9451S 1000 GPR120 Santa Curz sc-390752 1000 GPR40 Abcam 2478S 1000 ZO-1 Abcam ab96587 1000 Occludin Abcam ab31721 1000 Claudin-1 Abcam ab15098 1000 FASN Abcam ab99539 1000 ACACA Abcam ab72046 1000 FABP4 Abcam ab92501 1000 FATP4 Sangon Biotech D123165 1000 DGAT1 Abcam ab181180 1000 SREBP1 Abcam ab3259 1000 PPARγ Abcam ab178860 1000 CD36 Abcam ab133625 1000 GPR40 Abcam ab236285 1000 GPR120 Santa Curz sc-39752 1000 α-casein Santa Curz sc365929 100 β-casein Santa Curz sc166520 100 WAP Santa Curz sc398276 100 JAK2 CST 3230 1000 Phospho-JAK2 CST 3776 1000 STAT5 CST 94205 1000 Phospho-STAT5 CST 4322 1000 β-actin Abcam ab8226 2000 Goat Anti-Rabbit IgG Zenbio 511203 5000 Goat Anti-Mouse IgG Zenbio 511103 5000 Goat Anti-mouse IgG (H+L) Boster BA1126 100
Techniques: Staining, Concentration Assay, Western Blot, Fluorescence, Microscopy